高效液相色谱法测定痧气丸中天麻素的含量

Determination of Gastrodin in Shaqi Pills by High Performance Chromatography

目的:建立高效液相色谱法测定痧气丸中天麻素的含量。方法:采用shim-pack CLC-ODS(6mm×150mm)色谱柱,流动相:甲醇-磷酸盐溶液(0.1mol/L磷酸二氢钾溶液和0.1mol/L磷酸氢二钠溶液等量混合)-水(2:3:95),流速1.0mL/min,柱温35℃,进样量10μL,检测波长220nm。结果:天麻素在进样量0.01～0.05mg/mL范围内线性关系良好,回归方程为Y=794640X+393.2(r=0.9997,n=5),平均加样回收率为98.50%,RSD为1.95%。结论:本方法快速、简便、结果准确,可用于控制该制剂的质量。

Objective：To establish a high performance chromatography （HPLC） method to assay the concentration of gastrodin in Shaqi pills. Methods： Shim-pack CLC-ODS （6 mm× 150 mm） column and a mobile phase of methanol-phosphate solution （0.1 mol/L KH2PO4 solution and 0.1 mol/L Na2HPO4 solution contour mixed） -water （2： 3：95 ） were used with the flow rate of 1.0 mL/min and the column temperature of 35 ℃. The detection wave length was 220 nm. Results： There was a linear relationship within the range of 0.01 - 0.05 mg/mL and the regression equation was Y = 794640X ＋ 393.2 （ r = 0. 9997, n = 5 ）. The average relative recovery rate was 98.50% and RSD was 1.95%. Conclusions： This method is simple and accurate and able to control the quality of the preparation.