利钠肽受体-A基因敲除小鼠的繁育与鉴定

Breeding and Identification of Natriuretic Peptide Receptor-A gene Knockouted Mice

目的:繁殖和鉴定NPR-A基因敲除小鼠。方法:将所引进的雄性野生型和雌性NPR-A基因敲除杂合子小鼠进行交配繁殖,可以得到野生子、杂合子及纯合子3种基因型,提取每只小鼠尾部基因组DNA,用PCR法进行基因型鉴定。结果:NPR-A基因敲除小鼠的饲养和繁殖均获得成功,成功获得了较多的NPR-A基因敲除纯合子小鼠。结论:雌、雄性NPR-A基因敲除杂合子小鼠交配是繁育NPR-A基因敲除纯合子子鼠的较好方法;用PCR方法能够精确鉴定NPR-A基因敲除纯合子子鼠。

Objective：To breed and identify natriuretic peptide receptor- A （NPR-A） knock-out mice.Methods： Heterozygote mice were bred and reproduced.Wild genotype,heterozygote genotype,and homozygote genotype would appear in offsprings of parents.Genome DNA extracted from the mice tails was subjected to PCR for genotype identification.Results：The experimental model for NPR-A knock-out mice was successfully established and more NPR-A knock-out mice were reproduced.Conclusion： Appropriate methods for breeding,reproducing,and identifying are the effective way for acquiring NPR-A knock-out mice from heterozygote mice.