不同分子量的高纯度透明质酸对猪去全厚皮后伤口愈合的影响

THE EFFECT OF ULTRAPURE HYALURONIC ACID WITH DIFFERENT MOLECULAR WEIGHTS ON THE HEALING OF PORCINE FULL THICKNESS SKIN WOUND

透明质酸（ＨＡ）是一种大分子量的细胞外基质，它可影响伤口愈合。ＨＡ的作用与其分子量大小，以及是否含其它蛋白质有关。在猪去全厚皮伤口局部应用不同分子量的高纯度ＨＡ７天，与中分子量的ＨＡ（１００ｋｕ）和生理盐水对照相比，高分子量的ＨＡ（大于１０００ｋｕ）促进伤口的早期收缩，而低分子量ＨＡ（１０ｋｕ）则对伤口愈合有抑制作用。检测２１天的伤口抗撕裂强度，高、中分子量的ＨＡ有抑制作用，低分子量ＨＡ无作用。用激光多普勒扫描技术检测伤口血运动态变化，高、中分子量ＨＡ使第３天伤口血运下降，但在第７天，全部伤口血运增加。实验揭示了不同分子量的ＨＡ对伤口收缩、肉芽组织血管生成和伤口愈合强度的作用不一。证明，ＨＡ可用于伤口治疗或作为载体。

Hyaluronic acid (HA) is an extracellular matrix having a high molecular weight. This study is to investigate (1) whether the ultrapure HA had effect on the healing of full thickness skin wounds in animals, and (2) the relation between the effect of HA and the size of the molecular weight of HA. The ultrapure HA with different molecular weights and HA with intermediate molecular weight were applied topically to the wounds from removal of full thickness of skin and topical application of normal saline was used as control. At 21st. day after the topical application, the tensile strength was reduced in wounds of groups of HA with high and intermediate molecular weights, but not in the group of low molecular weight. Using laserDoppler scanning of the dynamic changes of the wound perfusion, in the 3rd day the blood supply to the wound was reduced in HA groups of high and intermediate molecular weights, but at the 7th day, the blood supply of all wounds in the two groups was increased. The study revealed that HA with different molecular weights had different actions on the contraction of wounds, the angiogenesis of the granulation tissues and the healing strength of the wounds. It was indicated that hyaluronan could be used for wound treatment or as a carrier, but its accurate composition must be carefully noted.