血小板源性伤口愈合因子对伤口组织细胞增殖作用的实验研究

EXPERIMENTAL STUDY OF PROLIFERATION EFFECTS OF PLATELET DERIVED WOUND HEALING FACTOR ON TISSUE CELLS OF WOUND

为了观察不同浓度的血小板源性伤口愈合因子（ＰＤＷＨＦ）对伤口成纤维细胞及表皮细胞增殖能力的影响，并探讨其可能的机理，利用离体培养的鼠伤口成纤维细胞及人伤口表皮细胞为模型，将培养细胞分为三组，空白对照组：基质处理组（ＢＭ，成分为１６４０＋０．５％ＦＣＳ）；阳性对照组：１６４０＋１０％ＦＣＳ；ＰＤＷＨＦ组：ＢＭ＋１％ＰＤＷＨＦ、ＢＭ＋３％ＰＤＷＨＦ、ＢＭ＋５％ＰＤＷＨＦ、ＢＭ＋７％ＰＤＷＨＦ、ＢＭ＋１０％ＰＤＷＨＦ及ＢＭ＋１２％ＰＤＷＨＦ。待测样本与细胞共孵育４８小时后，ＭＴＴ法测定细胞的增殖情况。结果表明，ＰＤＷＨＦ的浓度为１％～７％时，可显著促进伤口成纤维细胞及表皮细胞的增殖，明显高于０．５％ＦＣＳ组（Ｐ＜０．０１）；ＰＤＷＨＦ的浓度为１０％时，其促增殖作用已不再明显，与０．５％ＦＣＳ组无显著差异（Ｐ＞０．０５）；ＰＤＷＨＦ的浓度为１２％时，其只对伤口成纤维细胞的增殖呈现出明显抑制作用。认为，ＰＤＷＨＦ是多种生长因子的混合物，在一定的浓度范围内对伤口成纤维细胞及表皮细胞有明显的促增殖作用，但当其浓度过高时促增殖作用不明显。

This experiment was designed to observe the proliferative effects of platelet derived wound healing factor (PDWHF) of different concentrations on fibroblasts from rat wounds and on epithelial from human wounds. Cultured fibroblasts from rat wound and epithelia from human wound were randomly divided into three groups. (1) In blank control, the cells were treated with basic medium (BM, contains 1640/0.5% FCS); (2) the positive control, the cells were treated with 1640/10% FCS and (3)in the PDWHF group, the cells were treated respectively with BM/1% PDWHF, BM/3% PDWHF, BM/5% PDWHF, BM/7% PDWHF, BM/10% PDWHF, BM/12% PDWHF, respectively. The Cells were collected after 48 hours culturing with BM or PDWHF, and the cell proliferation was measured by MTT method according to the OD values. The result showed that the PDWHF could remarkably enhance the proliferation of fibroblasts and epithelial cells when its concentration was between 1% and 7%, which was obviously higher than that of the blank control (P<0.01). When the concentration of PDWHF reached 10%, its proliferative effect was not remarkable when compared with the blank control, When the concentration of PDWHF reached 12%, it showed inhibitory effect on fibroblasts and manifested no obvious inhibitory effect on epithelial cells. It was concluded that the PDWHF was a combination of a variety of growth factors. In a certain range of concentration, the PDWHF might effectively promote the proliferation of fibroblasts and epithelial cells. Howerve, when its concentration reached to relatively higher level, its effect was not remarkable any more, or even showed inhibitory effect on cell proliferation.