肿瘤坏死因子-α、白细胞介素-6与溃疡性结肠炎的关系术

Relationship between Tumor Necrosis Factor-α, Interleukin-6 and Ulcerative Colitis

背景：部分研究显示细胞因子改变在溃疡性结肠炎（UC）的发生机制中可能起重要作用。不同的细胞因子产物与基因启动子、信号序列、基因内含子中的单核苷酸多态现象有关。目的：测定UC患者肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的基因多态性及其血清浓度，从而探讨TNF-α、IL-6与UC的关系。方法：以聚合酶链反应-序列特异性引物（PCR—SSP）法检测60例UC患者和60名正常对照者的TNF-α、IL-6基因型，以酶联免疫吸附测定（ELISA）检测血清TNF-α、IL-6浓度。结果：UC患者TNF-α—308、IL-6—174位点基因型频率和等位基因频率与正常对照组相比均无明显差异；UC病变位于全结肠者的TNF-α-308GG基因型频率显著高于左半结肠组和直肠组（P＜0．05）。UC患者血清TNF-α、IL-6浓度显著高于正常对照组（P＜0．05）；UC患者不同基因型间血清TNF-α和IL-6浓度相比无明显差异，而正常对照组之间差异有统计学意义（P＜0．05）。结论：TNF-α-308、IL-6—174位点基因多态性与UC发病的易感性无关；TNF-α-308GG基因型可能与UC病变范围有关；健康人中TNF-α、IL-6基因型是其血清水平的决定性因素，而UC患者血清TNF-α、IL-6含量可能还受其他因素的影响。

Background：Some research data indicate that alterations of cytokine synthesis may play an important role in the pathogenesis of ulcerative colitis （UC）. Products of different cytokines have been linked to single nucleotide polymorphisms in gene promoter regions, signal sequences and gcne introns. Aims： To investigate the gene polymorphisms and serum levels of tumor necrosis faetor--α （TNF--α） and interleukin-6 （IL-6） in UC patients, so as to explore the relationship between TNF--α, IL-6 and UC. Methods： The genotypes of TNF--α and IL-6 in 60 UC patients and 60 normal controls were determined by polymerase chain reaction-sequence specific primers （PCR-SSP）. The serum levels of TNF--α and IL-6 were measured by enzyme-linked immunosorbent assay （ELISA）. Results： The genotypic frequency and allelic frequency of TNF--α-308 and IL-6-174 in UC patients had no significant differences compared with those in normal controls. The genotypic frequency of TNF--α-308GG in UC patients with pancolitis was significantly higher than that in left-sided and proctitis colitis （P〈0.05）. The serum levels of TNF--α and IL-6 in UC patients were significantly higher than those in normal controls （P〈0.05）, but no significant difference was observed in UC patients with different genotypes, whereas significant difference was found in normal controls having different genotypes （P〈0.05）. Conclusions： The gene polymorphisms of TNF-α-308, IL-6-174 may not be correlated with the susceptibility of UC. Genotype of TNF-α-308GG may be correlated with the extensive lesion of the disease. Genotypes of TNF-α and IL-6 may be the determinants of their corresponding serum levels in healthy adult subjects, however, the serum levels of TNF-α and IL-6 in UC patients may also be influenced by other factors.