携Sialyl Lewis^X与携抗P-选择素单抗靶向超声微泡粘附性的对比研究

Assessment of targeted adhesion of the two ultrasound contrast agents:one with Sialyl Lewis^X and the other with anti-P-selectin monoclone antibody in vitro

目的对比评价携sialylLewis。与携抗P-选择素单抗靶向超声微泡粘附特性。方法采用“亲和素-生物素”桥接法构建携Sialyl Lewis、（MB-S）、携抗P-选择素单抗靶向超声微泡（MB-P）及携同型抗体微泡（MB-C）。MB-S、MB-P及MB-C以相同流速通过相应小鼠P-选择素Fc段包被培养皿时，利用平行板流动腔在不同时间点测定相应的MB-S、MB-P及MB-C（对照组）的结合数目、滚动数目以及解离时达到半数解离的剪切应力。结果MB-S结合数量前3min快速增加，其后随时间增加无明显变化，而MB-P结合数量与时间呈正相关（P〈0．05），且MB-S结合数目是MB-P的2～4倍；对照组MB-C未见明显结合（P〈0．05）。MB-S滚动数目大于MB-P（P〈0．05）；MB-S半数解离时剪切力小于MB-P（P〈0．05）。结论靶向超声微泡MB-S表现为早期、快速、不稳定的结合及滚动，MB-P表现为缓慢牢固结合。

Objective To evaluate the adhesion effection of Sialyl Lewisx and anti - P - selectin monoclonal antibody targeted ultrasound microbubbles by parallel plate flow chamber. Methods The Sialyl Lewis^X （MB- S）, anti- P- selectin monoclone antibody （MB- P） and isotype IgG1 （MB- C） were constructed by ＂avidin- biotin＂ respectively. The attachment and detachment of MB- S, MB - P and MB - C to P - selectin immobilized on a culture dish were assessed in a parallel - plate flow chamber. While the attachment of MB - C served as a control group. Results The bonding number of MB - S increased rapidly in first 3 min, then no obviously increased appeared in the rest time, and there was positive correlation between the bonding number of MB - P and time（P〈0.05）. While the bonding number of MB - S bound was 2 - 4 times more than the number of MB-P. There was no obviously bonding of MB - C in control group （P〈0.05 ）. The rolling number of MB - S was larger than that of MB - P （P〈0.05）. There was limited adherence of the control group to P-selectin. Conclusion The appearance of MB - S targeted ultrasound microbul）bles is bonding and rollingearly, rapidly and unsteadily, and the appearance of MB - P is bonding slowly and firmly,