摘要
目的:利用我们前期构建的溶瘤腺病毒SG600技术平台,研究其携带野生型p53基因对裸鼠胃癌移植瘤模型抗肿瘤活性。方法:克隆p53基因全长cDNA,插入到溶瘤腺病毒SG600基因组中,同时建立裸鼠胃癌移植瘤模型,体内观察SG600-p53对胃癌移植瘤模型的抗肿瘤疗效。结果:成功构建溶瘤腺病毒SG600-p53,裸鼠胃癌移植瘤的p53基因获增强表达。在胃癌移植瘤裸鼠模型中,经4周治疗,重组SG600-p53抑瘤率达75.59%,优于Ad-p53(45.00%)和SG600(56.95%)。结论:SG600-p53作为一种有效的抗肿瘤新型制剂,对胃癌移植瘤有很好的特异性抗肿瘤活性,可能具有良好的临床应用前景。
Objective：By using the oncolytic adenovirus SG600 constructed previously, the antitumor activity of the p53 gene- enhanced oncolytic adenovirus SG600-p53 was examined in gastric cancer xenografts in nude mice. Methods：The full-length p53 cD- NA was cloned and inserted into the genome of oncolytic adenovirus SG600. The antitumor activity of SG600-p53 was observed in a nude mice model of human gastric cancer. Results： SG600-p53 could selectively kill＇gastric cancer cells, and inhibit the growth of gas- tric cancer xenografts in nude mice, with the tumor inhibition rate of 75.59%. Condusion：SG600-p53 is a novel effective antitumor agent with better antitumor activity in gastric cancer xenografts in nude mice, and may have a splendid future in clinic.
出处
《临床肿瘤学杂志》
CAS
2009年第3期193-195,共3页
Chinese Clinical Oncology
基金
国家自然科学基金资助项目(30572149,30700333)
