摘要
目的:探讨晚期食管癌患者化疗前后外周血中CD4+ CD25high调节性T细胞及Foxp3 mRNA的表达变化及其临床意义。方法:采用流式细胞术检测68例晚期食管癌患者化疗前后外周血CD4+ CD25high调节性T细胞的水平,并与40例健康成人进行比较。同时运用RT-PCR方法检测其中40例患者化疗前后外周血调节性T细胞Foxp3基因mRNA的表达情况。结果:(1)食管癌患者外周血中CD4+ CD25high调节性T细胞占CD4+T细胞总数的(1.82±0.54)%,显著高于健康对照组的(1.52±0.70)%(P<0.01);(2)化疗前患者外周血中CD4+ CD25high调节性T细胞为(1.82±0.54)%,明显高于化疗后的(1.66±0.58)%(P<0.05);(3)化疗前患者调节性T细胞Foxp3基因mRNA的相对表达量为0.318±0.027,明显高于化疗后的0.266±0.028(P<0.05)。结论:食管癌患者接受化疗后外周血中Foxp3 mRNA水平与CD4+ CD25high调节性T细胞数量表达均显著下降,推测Foxp3基因可能对CD4+ CD25highT细胞有重要的调节功能,从而影响食管癌患者化疗后CD4+ CD25high调节性T细胞的水平。
Objective：To explore the changes of CD4＋CD25high regulatory T cells and Foxp3 mRNA in patients with advanced esophageal cancer and its clinical significance. Methods：The frequency of CD4＋CD25high regulatory T cells in peripheral blood of 68 patients with advanced esophageal cancer and 40 healthy controls was determined by flow cytometry, the expression of Foxp3 mRNA in these regulatory T cells of 40 patients with advanced esophageal cancer were detected by RT-PCR. Results：CD4＋CD25high regulatory T ceils numbers were significantly more in 68 patients with advanced esophageal cancer （ CD4＋ T cells） than in 40 healthy donors. The level of CD4＋CD25high T cells was more in advanced esophageal cancer patients treated with chemotheraphy than in those patients with- out chemotheraphy. The level of Foxp3 mRNA were significantly less in advanced esophageal cancer patients treated with chemotheraphy than in those patients without chemotheraphy. Conclusion：As the level of CD4＋CD25highT cells, the level of Foxp3 mRNA significantly droped in advanced esophageal cancer patients treated with chemotheraphy, and Foxp3 genes may play a pivotal role in regulating the function of Treg cells and the level of CD4＋CD25highT in advanced esophageal cancer patients treated with chemotheraphy.
出处
《临床肿瘤学杂志》
CAS
2009年第3期196-198,共3页
Chinese Clinical Oncology
基金
安徽省科技厅重点科研计划资助项目(06023090C)
安徽省自然科学基金资助项目(007413256X)
