动脉粥样硬化与12/15-LOX基因启动子区甲基化状态的关系

The relationship between atherosclerosis and the status of 12/15-lipoxygenase gene promoter methylation

目的探讨动脉粥样硬化(AS)与12/15-脂氧合酶(12/15-LOX)基因启动子区甲基化状态的关系。方法将40只SD大鼠随机分为两组,即模型组和对照组,每组20只。模型组:给予高脂+维生素D3粉剂饮食;对照组正常饮食。3个月后,处死大鼠,取其主动脉弓至腹主动脉的血管组织,用改进的半定量甲基化特异性PCR法检测血管组织中12/15-LOX基因启动子区甲基化状态。结果①经苏木精-伊红染色,显微镜下观察可见模型组大鼠的主动脉有明显的粥样斑块形成,对照组未见明显变化。②按照吸光度(A)比值把全部血管组织分为低甲基化状态和高甲基化状态,模型组大鼠的血管组织的低甲基化率显著高于对照组(P<0.01)。结论血管斑块处12/15-LOX基因启动子区的甲基化状态与AS的形成相关,在AS发病过程中可能发挥着重要的作用。

AIM To explore whether atherosclerosis is related with the status of 15-lipoxygenase gene promoter methylation. METHODS 40 SD rats were randomly divided into two groups： experimental group and control group, 20 rats in each group. Feed the rats of experimental group by high-lipid and vitamin D3 overload for three months, while the rats of control group were normaly fed. Half-qualified methylation specific PCR was used to analyze the status of 15-1ipoxygenase gene promoter of vascular tissue from aortic arch to abdominal aorta in the rats. And calculate the A value of the methylation speci- ficity strap and the non-methylation specificity strap. RESULTS （~）Afier HE staining, obvious athero- matous plaque has been formed in the experimental group, but there is no histopathology changes in the control group. （~）The experimental group and control group were divided respectively into hypomethylated status and hypermethylated status. The incidence of experimental group in hypomethylated status（85% ） is significantly higher than in the control group （25 % ）. CONCLUTION The status of 15-lipoxygenase gene promoter methylation of the vascular tissue is related with the formation of atheroselerosis, and may play an important role in the pathogenesis of atherosclerosis.