醛固酮诱导的心肌重构及非洛地平的干预作用

Felodipine attenuates myocardial remodeling induced by aldosterone

目的研究非洛地平(felodipine,Fel)在醛固酮(aldosterone,Ald)诱导的心肌重构中的保护作用及其可能的机制。方法SD大鼠32只随机分为4组(每组n=8),即对照组、Ald组、Ald+Fel组和Ald+螺内酯(spironolac-tone,Spi)组。Ald组:以Ald 18μg/d皮下注射,连续4周;对照组:注射等量生理盐水4周;Ald+Fel组:以Ald18μg/d皮下注射,同时给于Fel 5 mg/(kg.d)灌胃,连续4周;Ald+Spi组:以Ald 18μg/d皮下注射,同时给予Spi20 mg/(kg.d)灌胃,连续4周。比较各组大鼠的左心室质量(LVW)及全心质量(HW)与体质量(BW)的比值,心肌组织匀浆超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,心肌胶原面积(CA),以及心肌细胞凋亡指数(AI)。结果与Ald组相比,对照组、Ald+Fel组和Ald+Spi组大鼠的LVW/BW,HW/BW,CA和AI均减少,对照组和Ald+Spi组大鼠MDA的含量较Ald组减少,SOD的含量较Ald组增加(P<0.01);Ald+Fel组大鼠SOD和MDA的含量与Ald组相比无明显差异。结论Fel能够改善Ald诱导的心肌肥大和心肌细胞凋亡,但不能改善Ald诱导的氧化应激水平的增加。

AIM To investigated the protective effects of felodipine （Fel） in aldosterone （Ald） induced myocardial remodeling. METHONDS Thirty-two rats were randomly assigned into 4 groups, 8 rats in each group： namely, Control group, Aid group Aid 18 μg/d hypodermic injection for 4 weeks）, Aid ＋ Fel group [ Ald 18 μg/d hypodermic injection and Fel 5 mg/（kg·d） intragastric administration for 4 weeks], and Aid ＋ Spi group （Ald 18μg/d hypodermic injection and Spi 20 mg/（kg·d） intragastric administration for 4 weeks）. All the animals were sacrificed after 4 weeks and the ratio of left ventricular weight/body weight （LVW/BW） and heart weight/body weight （HW/BW）, the concentration of SOD and MDA in cardiomyocyte homogenate, collagen area （CA） and apoptosis index （AI） were analyzed. RESUSTS Compared with those in Ald group, LVW/BW, HW/BW, CA and AI were lower in control group, Ald ＋ Fel group and Ald＋ Spi group. SOD was higher and MDA was lower in control group and Aid ＋ Spi group （ P 〈 0. 01 ）, but not in Ald ＋ Fel group. CONCLUSION Felodipine can improve aldosterone-induced myocardial hypertrophy and cardiomyocyte apoptosis but not aldosterone-induced oxidative stress.