趋化因子CXCL12及其受体在人前列腺癌嗜神经过程中的表达及相关机制

The Expression of CXCL12 and Its Receptor and Related Mechanism in Prostate Cancer Perineural Invasion

目的:阐述CXCL12-CXCR4在前列腺癌嗜神经过程中的作用及相关机制。方法:H&E染色观察前列腺癌细胞对肿瘤组织及其周围神经的浸润,免疫组织化学方法检测CXCL12和CXCR4、MMP-2、MMP-9在22例人前列腺癌和20例前列腺增生组织中的表达差别,浸润神经的肿瘤细胞CXCR4和CXCL12的表达。结果:在人前列腺癌组织中,存在着较明显的肿瘤细胞侵犯神经现象,人前列腺癌组织中CXCL12、CXCR4、MMP-2和MMP-9表达阳性率均较前列腺增生组织明显升高(P<0.05),侵犯神经的肿瘤细胞表达CXCR4,神经组织内神经鞘膜细胞表达CXCL12。结论:在前列腺癌组织中存在肿瘤细胞嗜神经现象,肿瘤肿瘤细胞分泌CXCL12,CXCR4与MMP-2、MMP-9促进了癌细胞的嗜神经浸润。

Objective： To identify the roles and related mechanism of CXCL12 and CXCR4 in perineural invasion of prostate cancer. Methods： Perineural invasion of prostate cancer cells was observed on H＆E stained sections from 22 cases of prostate cancer. A total of 22 prostate cancer cases and 20 prostate hyperplasia cases were involved in this study. The expression of CXCL12, CXCR4, MMP-2 and MMP-9 in nerve tissues and cancer cells that had invaded the perineural space were detected by immunohistochemistry. Resalts： Perineural invasion was a common phenomenon in human prostate cancer. The positive expression rates of CXCL12, CXCR4, MMP-2 and MMP-9 in cancer tissues were significantly higher than those in prostate hyperplastic tissues （P〈0.05）. Cancer cells invading the perineural space expressed a certain amount of CXCR4 and Schwann cells expressed a certain amount of CXCL12. Conclusion： Patients with prostate cancer may have perineural invasion. CXCL12, CXCR4, MMP-2 and MMP-9 secreted by cancer cells and CXCL12 secreted by Schwann cells promote perineural invasion of prostate cancer.