SERCA抑制剂抑制人结肠癌SW480细胞生长的作用机制研究

Detection of intracellular calcium concentration and apoptosis in Thapsigargin-induced human colon cell line SW480

目的探讨SERCA抑制剂抑制结肠癌细胞株SW480生长的作用机制，为结肠癌治疗提供实验依据。方法（1）应用Ca^2＋荧光指示剂Fura-2检测细胞内Ca^2＋的浓度。（2）MTT法检测细胞抑制率。结果毒胡萝卜素（Tg）可使SW480细胞内Ca^2＋水平由（133．26±5．17，197．46±4．62）明显升高到（436．63±5．21，766．41±3．12），差异有统计学意义（P〈0．05）；毒胡萝卜素（Tg）对人结肠癌细胞株SW480的抑制率随浓度（10～50μmol／L）的提高而增高，由（10．67±5．28）％至（31．46±7．21）％，抑制率差异有统计学意义（P〈0．05）；毒胡萝卜素（Tg）与W7合用时，这种效应则受到抑制。结论随着毒胡萝卜紊（Tg）浓度的增大，细胞内钙浓度同步上升；同时毒胡萝卜素（Tg）对该细胞株的抑制率增高。但其所诱导的细胞抑制具体钙信号途径还有待进一步研究。

Objective To investigate the mechanism of Thapsigargin in the proliferation of SW480 and provide experimental basis for development of anti-signaling therapy for patients with colon cancer by detection of intracellular calcium concentration in Thapsigargininduced human colon cell line SW480,. Methods Level of [Ca^2＋] was measured by fluorescence measurement with fura-2/AM, a fluorescent index of calcium. The suppression rate of SW480 ceils was calculated by MTT assay. Results Thapsigargin promoted the levels of [Ca^2＋] from （ 133.26±5.17,197.46±4. 62）to（436. 63±5.21,766. 41±3.12 ）, and inereased the suppression rate from （ 10.67±5.28） to （ 31.46±7.21 ）. When W7 was combined used with Thapsigargin, no significant changes were found in the levels of [Ca^2＋] and increment of the suppression rate. Conclusion Thapsigargin might promote the proliferation of SW480 cells and lead to apoptosis, but the signaling pathway still remain to be studied.