摘要
目的探讨Toll样受体4(TLR4)与冠状动脉粥样硬化(AS)斑块急性病变的关系。方法从本教研室2004～2007年尸检档案中挑选24例病例及其心脏标本。分为实验组(有冠脉急性病变的SCD者)、对照组Ⅰ(无冠脉急性病变的SCD者)和对照组Ⅱ(冠脉无严重AS的暴力死者)等3组,每组8例。用免疫组化SABC法和图像分析技术定量检测TLR4在各组冠状动脉AS斑块内表达的平均光密度值(OD),所得数据用SPSS14.0统计学软件进行分析处理并比较各组间的差异。结果实验组冠状动脉AS斑块中TLR4表达呈强阳性,对照组Ⅰ呈相对较弱的阳性表达,而对照组Ⅱ冠状动脉壁及轻度增厚的内膜处均未见阳性表达,其OD值(0.4662±0.0190)与两个对照组(分别为0.3361±0.0165,0.2463±0.0190)相比,对照组Ⅰ与对照组Ⅱ相比,其差异均具有统计学意义(P<0.01)。结论冠状动脉AS斑块中TLR4的表达情况可视作为冠状动脉AS斑块发生急性病变的一个病理诊断指标,也是诊断冠心病猝死的重要依据之一。
Objective To investigate the TLR4 expression in the coronary atherosclerotic plaque with acute coronary artery lesions and its relationship with the acute coronary artery lesions. Methods Twenty- four cases and heart samples autopsied in our department from 2004 to 2007 were selected and divided into 3 groups of the experimental group, control group A and control group B （8 eases in each group）. The TLR4 expressive change in the coronary atherosclerotic plaque of each group and their average optical densities （OD values） were detected by the immunohistochemistry （SABC method ） and image analysis technology. All data and the difference among groups were analyzed statistically. Results The strong posi- tive expression of TLR4 was found in the experimental group, positive expression in the control group A, and negative expression in the control group B. The result of statistical analysis indicated that the difference of OD value of the TLR4 expression in the coronary atherosclerotic plaques was of statistical significance between the experimental group （0. 4662 ± 0. 0190 ） and the two control groups （0. 3361 ±0. 0165,0. 2463 ±0.0190） respectively, and between the control group A and B. Conclusion The expressive change of TLR4 in the coronary atherosclerotic plaque can be regard as an important marker of pathological diagnosis of acute coronary artery lesions, and as an important evidence to diagnose sudden coronary death.
出处
《中国法医学杂志》
CSCD
北大核心
2009年第2期77-79,I0001,共4页
Chinese Journal of Forensic Medicine
