SD大鼠颌下腺腺泡细胞培养及胶原海绵的细胞相容性

Cell Culture of Submandibular Gland from SD Rats and Its Compatibility with Collagen Sponge Scaffold

【目的】研究SD大鼠颌下腺腺泡细胞体外培养方法以及和胶原海绵的细胞相容性,探讨胶原海绵应用于涎腺组织工程支架材料的可行性。【方法】取新生SD大鼠颌下腺组织,胰酶消化后将细胞于适宜的体外环境培养、纯化、鉴定,并将鉴定好的颌下腺腺泡细胞移植到胶原海绵上,采用DAPI进行特异性的核染色,荧光显微镜下观察腺泡细胞与胶原海绵的相容情况,评价腺泡细胞与胶原海绵的相容性。【结果】体外培养的颌下腺腺泡细胞经纯化后能够分泌特异性的淀粉酶,免疫细胞化学淀粉酶抗体阳性染色,并能在胶原海绵上正常增殖。【结论】体外培养的颌下腺腺泡细胞具备腺泡细胞的生物学特征并与胶原海绵有很好的相容性,有望将胶原海绵作为组织工程化涎腺组织的支架材料之一。

[ Objective ] To study in vitro culture of submandibular gland acinar cells （SMGC） isolated from SD rats and the compatibility between SMGC and collagen sponges, and to investigate the feasibility of collagen sponges applied to the scaffold in salivary tissue engineering. [ Methods ] The submandibular gland tissues isolated from neonatal SD rats were digested into SMGC by pancreatic enzyme. After cultured, purified, and identified in vitro, the identified SMGC were transplanted into the collagen sponges. Then the SMGC were stained in nuclear by 4＇, 6-diamidino-2-phenylindole dihydrochloride staining （DAPI） every week and observed under fluorescence microscope. The compatibility between SMGC and collagen sponges was evaluated. [ Results ] The SMGC cultured in vitro could secrete specific amylase after purification. Positive immunocytochemieal staining of amylase in SMGC could be observed. SMGC could proliferate well in collagen sponges. [ Conclusions] SMGC cultured in vitro have the biological characteristics of acinar cells and the compatibility with collagen sponges. Collagen sponge can be served as one of the salivary tissue engineering scaffolds.