硫酸铵盐析法分离罗非鱼肌红蛋白的研究

Separation of myoglobin from tilapia by ammonium sulfate salting-out

通过测定盐析液的色泽、肌红蛋白含量、纯度、回收率和吸收光谱等的变化情况,分析了硫酸铵分级盐析过程对罗非鱼肌红蛋白分离的影响。研究表明,硫酸铵饱和度为60%～100%可导致肌红蛋白沉淀析出,其中饱和度为50%时,肌红蛋白纯度为37.52%,回收率仅为39.89%;饱和度大于60%时,肌红蛋白纯度虽有所降低,但回收率逐渐提高,当饱和度为100%时,回收率最高为87.56%。在盐析过程中,部分氧合肌红蛋白氧化成为高铁肌红蛋白。

Effect of ammonium sulfate on salting-out of myoglobin was studied by analysing color, Mb content, Mb purity, recovery rate and absorption spectra. The results showed that the myoglobin could be precipitated after treating with ammonium sulfate at saturation 60% - 100% , and the purity and recovery rate were 37.52% and 39. 89% respectively after treated with 50% ammonium sulfate. Mb purity was slightly decreased but the recovery rate was increased gradually when the saturation was greater than 60%. And recovery rate was up to 87. 56% when the saturation reached to 100%. Oxymyoglobin was partly oxidated into the met-myoglobin in the process of the ammonium sulfate salting-out.