氯化三丁基锡对黑鲷鳃和肝组织SOD、MDA和GPx的影响

Effects of tributyltin chloride (TBTCl) on SOD activities,MDA contents and GPx activities in gill and liver of the black porgy(Sparus macrocephalus)

观察和比较了黑鲷Sparus macrocephalus在不同浓度的氯化三丁基锡(tri-n-butyltin chloride,TBTCl)溶液中暴露后,其鳃和肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)活性的变化情况。结果表明,TBTCl对黑鲷鳃SOD活性有抑制作用,对肝脏SOD活性表现出促进作用;暴露TBTCl溶液中24h后,黑鲷鳃和肝脏MDA含量均有升高,且随浓度变化与对照组比较有显著性差异(P<0.05),但暴露48h后,2组织中MDA含量相对于对照组均降低。TBTCl对黑鲷鳃GPx活性的影响先是表现出显著性抑制,之后随时间延长呈显著的促进效应,肝脏则呈现随浓度增加GPx活性被显著性地促进的趋势。黑鲷体内鳃和肝脏组织中抗氧化酶系统对TBTCl的毒理学敏感性,表明其可以作为监测有机锡污染程度的效应指标。

In this study, the malondialdehyde （MDA） content, the glutathione peroxidase （GPx） activity, and the superoxide dismutase （SOD） activity in black porgy Sparus macrocephalus were determined after the acute exposure to TBTCl at the concentration of 4 and 20 μg·L^-1. Results showed that TBTCl inhibited the SOD activity in S. macrocephalus gill slightly, but there was an obvious pro-motion on the SOD activity in S. macrocephalus liver. After 24 h exposure to TBTCl, the MDA contents in two tissues increased significandy （P 〈 0. 05 ） with the increase of the concentration of TBTCl compared with the control. However, the MDA contents decreased and were lower than the control after 48 h exposure. The effects of TBTCl on the GPx activity in S. rnacrocephalus had obvious difference between gill and liver; the GPx activity in gill was significantly inhibited after 24 h exposure, and then was promoted significantly with the increase of exposure time. There was a significant promotion in S. macrocephalus liver and reached the peak at the concentration of 20 μg·L^-1 TBTCl. The significant changes subjected to oxidative stress and damage exposed to TBTCl indicated that they could be used as the sensitive biomarker to monitor the pollution level of organic compounds.