东北梅花鹿卵泡发育过程中卵泡细胞超微结构的观察

Study on Ultrastructure of the Follicle Cells in the Development of Oocytes in Sika Deer

为了进一步弄清东北梅花鹿卵泡细胞超微结构的变化规律,本研究用透射电镜、目镜测微尺和显微照相技术对各期卵泡中卵泡细胞的超微结构进行了观察,并按卵泡大小、颗粒细胞层数、透明带状况及卵泡腔是否出现,把卵泡分成4个阶段。结果表明:原始卵泡-卵泡细胞与卵母细胞已开始建立结构上的联系,卵泡细胞的形态变化也是适应卵泡发育的需要;初级卵泡-细胞器数量增加,结构变得清晰,卵泡细胞中出现厚壁小泡,可能是一种早期的脂滴;次级卵泡-卵泡突起穿过透明带,并出现有营养作用的脂滴和支持作用的微丝;三级卵泡-高尔基复合体发达,多与粗面内质网相贴,核糖体丰富,成簇分布,接近排卵的卵泡中卵泡细胞发生扩展等一系列结构上的变化将利于受精。

In order to reveal the developmental progress of sika deer（Cerv us nippon） follicular cells, in this experiment follicle systems were divided into 4 sages according to the Cervus nippon＇s follicle diameter size, formation of zonapellucida, appearance time of follicular cavity, at the same time, observations on uhrastructure of follicular ceils in the developmental follicles were conducted with electron microscopy, eyepiece micrometer and photomicrographic technique. The results indicated that primordial follicular, the follicile cells were connected with the oocyte cells, the morphological changes of follicile cells was adapted to the development of follicile. Primary follicular, the number of organelles increased, the structure of organelles became clearer and the thick walled vesicles began to appear which would be precursor of lipid droplets. Secondary follicular, mitochondria was round or elliptic, which was often surrounded by rough endoplasmic reticulum. In this case, lipid droplet and microfilament were observed and would play great role in supporting and nourishing separately. Tertiary follicle, there were many Golgi complex, endoplasmic reticulum and ribosome. In the follicle of close ovulation, the cumulus expansion would be advantageous to fertilization.