期刊文献+

苦参碱对肺腺癌细胞生长抑制的实验研究 被引量:5

Growth Suppression Effect of Matrine on Human Lung Cancer Cell Lines in vitro
下载PDF
导出
摘要 目的:研究分化诱导剂苦参碱(Matrine)在体外对人肺腺癌SPC-A-1细胞生长抑制的影响。方法:用不同浓度的苦参碱分别处理肺腺癌SPC-A-1细胞后,镜下观察癌细胞的生长情况;测定软琼脂克隆形成率;MTT(噻唑蓝)法测定生长抑制率。结果:不同浓度苦参碱处理后肺腺癌SPC-A-1细胞的生长明显受到抑制,细胞生长抑制率随苦参碱的浓度增加而增强。结论:苦参碱对肺腺癌SPC-A-1细胞的生长有明显的抑制作用。 Objective: To study effect of Matrine on the growth suppression of human lung adenocarcinoma cell line SPC-A-1 in vitro. Methods: Different concentrations of Matrine were used in the culture of SPC-A-1 cells. Colony forming efficiency on soft agar was determined and the growth suppression rate was measured by tetrazolium-based volorimetric assay (MTT). Results: The growth of SPC-A-1 cells was markedly inhibited after the treatment of Matrine on different concentrations. The rate of growth suppression was increasing as the concentration of Matrine increased. Conclusion: Matrine has great effects on inhibiting the growth of human lung adenocarcinoma cell line SPC-A-1.
出处 《浙江中西医结合杂志》 2009年第4期207-208,215,共3页 Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金 福建省卫生厅青年科研课题(No.2007-2-50)
关键词 肺腺癌 SPC-A-1细胞 苦参碱 生长抑制 lung adenocarcinoma SPC-A-1 cell Matrine growth suppression
  • 相关文献

参考文献7

二级参考文献23

  • 1钟声,徐永健,张珍祥.CC10在苦参碱诱导的肺腺癌细胞凋亡中的表达[J].华中科技大学学报(医学版),2006,35(1):124-126. 被引量:7
  • 2刘伟,唐金花,王亚娣.苦参的研究进展[J].时珍国医国药,2006,17(5):829-830. 被引量:41
  • 3雷怀定,刘先军,涂明利,熊畅,刘为舜.苦参碱对体外人肺癌A549细胞的抑制作用[J].实用医学杂志,2006,22(15):1717-1719. 被引量:24
  • 4张慧芳,张煦,范临兰.鬼臼毒衍生物诱导人肺腺癌A549细胞凋亡[J].第四军医大学学报,2006,27(16):1516-1518. 被引量:7
  • 5李先荣.苦参生物碱抗小鼠移植性肿瘤研究[J].中西医结合杂志,1982,2(1):42-42.
  • 6[3]Zhang Yah,Jiang J K,Liu X S,et al.Differentiation and apoptosis inK562 erythroleukemia cells induced by matrine[J].Nat Med,1998,52(4):295-299.
  • 7肖诗鹰.苦参抗肿瘤方面的应用与研究[J].实用中西医结合杂志,1991,4(9):564-566.
  • 8Chen J,Ramos J,Sirisawad M,et al.Motexafin gadolinium induces mitochondrially-mediated caspase-dependent apoptosis[J].Apoptosis,2005,10(5):1131-1142.
  • 9Ly JD,Grubb DR,Lawen A,et al.The mitochondrial membrane potential (△ψm) in apoptosis; an update[J].Apoptosis,2003,8(2):115-128.
  • 10Metcalfe C,Thompson SG,Cowie MR,et al.The use of hospital admission data as a measure of outcome in clinical studies of heart failure[J].Eur Heart J,2003,24:105-107.

共引文献111

同被引文献65

引证文献5

二级引证文献33

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部