摘要
目的:研究分化诱导剂苦参碱(Matrine)在体外对人肺腺癌SPC-A-1细胞生长抑制的影响。方法:用不同浓度的苦参碱分别处理肺腺癌SPC-A-1细胞后,镜下观察癌细胞的生长情况;测定软琼脂克隆形成率;MTT(噻唑蓝)法测定生长抑制率。结果:不同浓度苦参碱处理后肺腺癌SPC-A-1细胞的生长明显受到抑制,细胞生长抑制率随苦参碱的浓度增加而增强。结论:苦参碱对肺腺癌SPC-A-1细胞的生长有明显的抑制作用。
Objective: To study effect of Matrine on the growth suppression of human lung adenocarcinoma cell line SPC-A-1 in vitro. Methods: Different concentrations of Matrine were used in the culture of SPC-A-1 cells. Colony forming efficiency on soft agar was determined and the growth suppression rate was measured by tetrazolium-based volorimetric assay (MTT). Results: The growth of SPC-A-1 cells was markedly inhibited after the treatment of Matrine on different concentrations. The rate of growth suppression was increasing as the concentration of Matrine increased. Conclusion: Matrine has great effects on inhibiting the growth of human lung adenocarcinoma cell line SPC-A-1.
出处
《浙江中西医结合杂志》
2009年第4期207-208,215,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
福建省卫生厅青年科研课题(No.2007-2-50)