摘要
目的建立测定追风药酒中橙皮苷含量的高效液相色谱(HPLC)法。方法色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相为甲醇-3%冰醋酸溶液(45:55),流速为1.0mL/min,检测波长为283nm,柱温为30℃。结果橙皮苷进样量在0.2932～1.7592μg范围内与峰面积线性关系良好,r=0.9998(n=6);平均加样回收率为99.12%,RSD为1.17%(n=6)。结论方法简便、准确、重现性好,可用于追风药酒的含量测定。
Objective To establish the determination method of hesperidin in Zhuifeng Medicinal Wine by HPLC. Methods HPLC was adopted with Kromasil C,s column（250 mm×4.6 mm, 5 μm） at the temperature of 30 ℃.The mobile phase consisted of methanol-3% glacial acetic acid（45： 55）with the flow rate of 1.0 mL/min. AUV- detector at 283 nm was used. Results Hesperidin showed good linear relationship in the range of 0.293 2-1.759 2 μg, r=0.999 8（n=6）.The average recovery rate was 99. 12%, RSD=1.17% （n=6）. Conclusion The method is simple and accurate with a good reproducibility. It can be used for the quality control of Zhuifeng Medical Wine.
出处
《中国药业》
CAS
2009年第7期19-20,共2页
China Pharmaceuticals
