淫羊藿总黄酮体外抗氧化实验

Anti-oxidation on Total Flavones of Epimedium in vitro

对淫羊藿有效成分总黄酮进行了体外抗氧化实验。离体培养大鼠肝组织,建立体外组织的过氧化模型,通过测定淫羊藿提取物对多种自由基的清除效果,以验证其抗氧化功能。其中对肝匀浆的自氧化及诱导性氧化均有较好的抑制作用,并成一定的对数曲线关系,其IC50分别为34.87ug/mL和198.45 ug/mL;对肝线粒体的诱导性氧化亦有较好的抑制作用,其IC50剂量为332.65 ug/mL;对DPPH、.OH、.O2-的清除作用IC50分别为4.67 ug/mL、598.17 ug/mL和413.21 ug/mL。结果表明:该提取物对大鼠肝组织及肝线粒体的自发和诱导的脂质过氧化均有很好的抑制效果;并能对超氧自由基、羟自由基及DPPH自由基进行清除,具有较好的抗氧化功能。

The anti-oxidation of TFE in vitro was measured.TFE was able to restrain the oxygen level of liver homogenate and mitochondrion either spontaneously or inductively,which IC50 were especially 34.87 μg·mL-1,198.45 μg·mL-1 and 332.65 μg·mL-1.332.65 μg·mL-1.Moreover,its effect was predominant in eliminating free radiations including DPPH,·OH and ·O2-,which IC50 were especially 4.67 μg·mL-1,598.17 μg·mL-1 and 413.21 μg·mL-1.