PCR技术检测金黄葡萄球菌肠毒素A基因

Study on Detection of Staphylococcus aureus Enterotoxin A Gene by PCR

目的建立一种快速、准确检测金黄葡萄球菌肠毒素A的PCR方法,了解引起临床感染的金黄葡萄球菌携带肠毒素A基因的情况。方法根据金黄葡萄球菌肠毒素A基因编码序列设计一对PCR引物来特异性扩增靶基因片段,建立快速、特异、灵敏的检测金黄葡萄球菌肠毒素A基因的方法。同时对我院2006年8月-2008年10月临床分离的120株金黄葡萄球菌进行肠毒素A基因检测。结果成功的对肠毒素A基因进行检测并进行基因测序。在我院120株受检金黄葡萄球菌中,基因阳性株占83.3%。结论用PCR技术检测金黄葡萄球菌肠毒素A基因具有特异性强,灵敏度高,速度快和易操作特点。金黄葡萄球菌肠毒素A阳性株在临床分离的金黄葡萄球菌中占有较高比例,应予以足够重视。

Objective To establish a rapid and specific assay for the detection of Staphylococcus aureus Enterotoxin A （SEA）. To survey the distribution of Enterotoxin A in Staphylococcus aureus （SA） isolated from clinical specimens. Methods According to the sequences of the SEA gene, primers were selected to amplify SEA gene, and a rapid, specific and sensitive method was established to detect SEA gene. Meanwhile, the SEA gene was detected in 120 strains of SA that isolated from clinical sources from August, 2006 to October, 2008. Results We detected the SEA gene and made the gene sequencing successfully. 100 of 120 strains of SA were positive （83.3 % ）. Conclusions The results suggest that PCR assay features high specificity and sensitivity, rapid speed, and easy operation in detection of the SEA gene. It is necessary to pay attention to the high proportion of SEA gene positive strains in clinical detection.