2008年衡阳市水产品霍乱弧菌监测及药敏结果报告

Monitoring and Drug Sensitivity Report of Vibrio cholerae in Aquatic Products from Hengyang Markets in 2008

目的对水产品中霍乱弧菌进行实验室鉴定、毒力基因(ctxA和TcpA)检测及药敏分析。方法细菌的分离、鉴定和药敏试验按照卫生部疾病控制司《霍乱防治手册》(第5版)1999提供的方法进行;用PCR方法对霍乱弧菌的ctxA和TcpA2种毒力基因进行检测。结果从277份水产品中检出1株小川型霍乱弧菌、1株稻叶型霍乱弧菌和7株O139血清群霍乱弧菌,阳性率3.25%;1株O139血清群霍乱弧菌毒力基因ctxA阳性,TcpA阴性,其它菌株的毒力基因ctxA和TcpA均为阴性;9株霍乱弧菌菌株对15种药物的敏感度完全一致,其中耐药的抗菌药物有复方新诺明、四环素、链霉素等3种,敏感的抗菌药物有诺氟沙星、环丙沙星、丁胺卡那霉素、庆大霉素等8种,中敏的抗菌药物有强力霉素、红霉素等4种。结论在霍乱防治工作中应密切监测水产品中霍乱弧菌的药物敏感性以及携带毒力基因的情况;诺氟沙星、环丙沙星、丁胺卡那霉素等抗菌药物可作为抗菌治疗和预防性服药的首选药物,而复方新诺明、四环素和链霉素则不应被选用。

Objective To detect and identify the ctxA and TcpA virulence genes, and to analyze their drug resistance of Vibrio cholerae. Methods Isolation, identification and drug sensitivity of V. cholerae were performed according to the Manual of V. cholerae published in 1999, and the virulence genes（ctxA and TcpA）of V. cholerae were amplified by PCR. Results 1 Ogawa V. cholerae, 1 Inaba V. cholerae O1 group, and 7 O139 groups were isolated from 277 aquatic products, with the positive rate of 3.25 %. In the ？ V. cholerae O139 groups, 1 was ctxA positive but TcpA negative, these two genes were negative in other strains, however. The drug sensitivity of these 9 V. cholerae strains to 15 kinds of drugs were identical, they were sensitive to norfloxacin,ciprofloxacin, amikacin, gentamycin and mild sensitive to vibramycin, erythromycin but resistant to chemitrim, tetracycline, streptomycin. Conclusions We should pay special attention to monitoring the drug sensitivity and virulence genes of V. cholerae in aquatic products. Norfloxacin, ciprofloxacin, amikacin are effective drugs for therapy and prevention of V. cholerae, but chemitrim, tetracycline and streptomycin are not recommended.