乌索酸抗实验性肝损伤作用与大鼠经口给药药动学研究

Protective Effects of Ursolic Acid on Mice or Rats Acute Hepatic Injury,and Pharmacokinetic Studies of Ursolic Acid in Rat Plasma After Administration of the Ursolic Acid

目的:乌索酸(熊果酸)是从中草药中提取分离得到的一个三萜类化合物,研究其灌胃给药对急性实验性肝损伤的保护作用和药动学特点。方法:采用急性CCl_4肝损伤模型、D-半乳糖胺盐酸盐大鼠急性肝损伤模型究乌索酸灌胃给药对急性实验性肝损伤的保护作用,并建立LC-MS分析方法检测乌索酸灌胃给药(80mg/kg)后大鼠血浆中乌索酸含量。结果:乌索酸对CCl_4致小鼠急性肝损伤及D-半乳糖胺盐酸盐致大鼠急性肝损伤没有保护作用,大鼠灌胃给予乌索酸后用LC-MS在血浆中未检测到乌索酸。结论:乌索酸经口给药由于吸收很差,故没有抗实验性肝损伤作用。

Objective： To investigate the protective effects of ursolic acid on mice or Ratsacute hepatie injury, and pharmacokinetic of ursolic acid in rat plasma after administration of the ursolic acid. Methods ： The liver-injury model was established by mice or rats acute hepatic injury induced by CCl4 or D-GaIN. A rapid, sensitive, and accurate liquid ehromatography-ma.gs speetrornetry（ LC-MS ） method for the determination of ursolie acid in rat plasma was developed and validatecl. Plasma samples taken from rats that had received ursolic acid orally. Results ： The ursolic acid didn＇t show protective effects oil mice or rats acute hepatic injury induced by CC14 or D-GaIN. The LC-MS method hasn＇t been suecessfully applied to pharmacokinetie studies of ursolic acid after oral administration of ursolie acid to rats. Conclusions： Because the ursolie acid is poorly absrbod by the intestine, it has not protective effects against chemical liver injuries.