福建漳江口红树林沉积物中的古菌类群

Archaeal lineages detected in mangrove sediment in the Zhangjiang estuary of Fujian province

利用环境16S rRNA基因序列分析技术,对福建漳江口红树林沉积物中存在的古菌类群进行检测。首先提取红树林沉积物的总DNA,并进一步构建环境16SrRNA基因克隆文库。随机挑选文库克隆,根据Amplified rDNAre-striction analysis(ARDRA)分析分型后进行测序。对所测序列进行系统进化分析。结果显示,文库中的大部分古菌克隆(95%)分别属于广古菌门(Euryarchaeota)中的Marine benthic group D和泉古菌门(Crenarchaeota)的Miscellaneous crenarchaeotic group和Marine group I等3个已描述的古菌类群,另有小部分克隆(5%)可能代表广古菌门中新的古菌类群。Miscellaneous crenarchaeotic group类群古菌是红树林沉积物中古菌的优势类群。上述古菌类群的检测加深了人们对于红树林古菌群落结构复杂性及其生态学意义的认识。

Archaeal lineages in mangrove sediment in the Zhangjiang estuary of Fujian province were detected by environmental 16S rRNA gene sequences phylogenetic analysis. Total DNAs were extracted from the mangrove sediment and an archaeal 16S rRNA gene clones hbrary was constructed. The archaeal 16S rRNA gene clones selected by amplified rDNA restriction analysis （ARDRA） were sequenced and phylogenetically analyzed. The results showed that most of archaeal 16S rRNA gene clones （95%） belonged to three described archaeal hneages, which were the marine benthic group D in the Euryarchaeota phylum, the miscellaneous crenarchaeotic group and the marine group Ⅰ in Crenarehaeota phylum. A few archaeal clones （5 % ） constituted partially novel hneages in the Euryarchaeota phylum. The miscellaneous crenarehaeotic group（MCG）crenarchaea was the predominant archaeal group in the mangrove sediment. The archaeal hneages revealed in this study enhanced our understanding of the ecological complexity and importance of archaea in mangrove sediment.