济宁青山羊大小猾子皮的差异表达基因研究

Studies on differential expressed genes between older and young goat skins of the Jining grey goat

以差异显示PCR(DDRT-PCR)技术研究了小猾皮(出生3d内)和大猾皮(出生30～60d)的差异表达基因,并以反Northern点杂交技术快速剔除假阳性条带。从40对随机引物中筛选出8对引物组合,其在2种猾子皮中共扩增出482条cDNA片段,其中差异表达条带为52条,占总条带数的10.79%。在所发现的差异条带中有41条为表达"强与弱"的差异带,占总条带数的8.51%;另有11条为表达"有或无"的差异条带,占总条带数的2.28%。说明与小猾皮相比,大猾皮皮肤组织中有8.51%的基因增强或减弱表达,有2.28%的基因启动或关闭表达。这些差异表达的基因是大猾皮和小猾皮花纹品质差异的遗传基础。

DDRT-PCR and reverse Northern dot hybridization methods were used to detect the different expressed genes between kid lambs（within 3 days old）and the older lambs skins （from 30 to 60 days old） of the Jining Grey Goat. The false bands were quickly rejected by reverse Northern dot hybridization. The results indicated that 482 cDNA fragments were isolated using 8 kinds of primer combinations selected from 40 pairs of random primer with DDRT-PCR. 52 differential expressed bands （ 10.79% ） were discovered, of which 41 bands （8.51%） were differentely expressed in amount between kid lambs＇ skin and those of the older lambs＇ skin, and there were 11 newly expressed or closed genes （2.28 % ） of those found. This means that compared to kid lambs, older lambs have 8.51% gene expressed difference in amount, and 2.28 % new genes appeared or closed in the skins. The differential expressed genes would be the genetic basis of figure quality between kid lambs and older lambs skins.