外周免疫活性淋巴细胞对致敏内耳的识别

Discernment of the sensitized inner ear by peripheral immunocompetent lymphocytes

目的了解外周免疫活性淋巴细胞在内耳免疫反应中的作用。方法提取全身致敏加双侧内耳致敏（Ａ组）、全身致敏（Ｂ组）和对照组（Ｃ组）等３组供者动物（豚鼠）的外周淋巴细胞，并在体外行５１Ｃｒ核素标记。将标记的淋巴细胞分别转移到具有相同免疫史的（即全身致敏加右侧内耳致敏）受体豚鼠内，按不同的供者来源，受体动物亦分为Ａ组（１５只）、Ｂ组（１０只）、Ｃ组（１０只）。２０～２４小时后取出受体动物双侧颞骨，称重，用γ计数器测定其放射性含量（ＣＰＭ／ｇ）。结果ＣＰＭ／ｇ平均值Ａ、Ｂ两组致敏耳（右耳７７７．３４和６９８．３５）明显高于非致敏耳（左耳６００．３２和５５３．４２）（Ｐ值均＜０．０５），Ｃ组两耳间差异无显著性（Ｐ＞０．０５）。Ａ组致敏内耳与非致敏内耳ＣＰＭ／ｇ的比率为１．３４，明显高于Ｂ组（１．１２）和Ｃ组（１．０２），差异有显著性（Ｐ＜０．０５）。结论内耳在抗原刺激下产生免疫反应受外周循环系统的免疫监控。

Objective To understand the role of peripheral immunocompetent lymphocytes during an immune response in the inner ear. Methods Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen from three groups of donor animals (group A was immunized intradermally with KLH and both inner ears were challenged with KLH; group B received only KLH intredermally without an inner ear challenge, and groups C were naive animals) were labelled with 51 Cr and injected intravenously into recipients (all of which were immunized intradermally with right inner ears challenged with KLH). Twenty to twenty four hours later, the temporal bones of recipients were assayed for radioactivity to detect the infiltration of labelled cells. Results In group A and B,the right temporal bones showed higher radioactivity than the left temporal bones ( P <0.05). The highest labelling was detected in the right temporal bones of group A. The lowest migration of 51 Cr labelled cells to the inner ear was found in group C animals. The highest ratios between right (challenged) and left (unchallenged) were found in group A animals compared with those of group B and group C. The value was significantly higher in group A than in group B and C ( P <0.05). Conclusion The results showed that the inner ear comes under the immunosurveillance of the peripheral circulation in response to antigenic stimulation.