摘要
目的研究神经生长因子(nervegrowthfactor,NGF)在面神经损伤修复中的作用。方法将新西兰家兔30只双侧面神经上颊支横断后置入硅胶神经再生腔,一侧注入NGF为实验侧,另侧注入生理盐水为对照侧。术后4周和8周各取15只家兔进行组织学观察及形态定量分析。结果术后4周,实验侧再生神经成功11支,神经髓鞘厚度0.779±0.475μm,有髓轴突计数共2024±1999;对照侧再生神经成功8支,髓鞘厚度0.413±0.132μm,有髓轴突计数共368±171,t检验两侧差异有显著性(P<0.05)。术后8周,再生神经更显成熟,再生神经有髓轴突直径、面积、髓鞘厚度及轴突计数均明显优于对照侧,两侧差异有显著性(P<0.05)。结论NGF在面神经损伤修复中具有促进作用。
Objective To elucidate the role of NGF in the regeneration of facial nerve. Methods The superior buccal division of facial nerve of adult New Zealand rabbit was transected and a nerve growth chamber created. The chamber of the experimental side was filled with NGF/normal saline and that of the control side with normal salinealone. Four and eight weeks after operation, the regenerated nerves in the chambers were dissected for histological studies. Results Four weeks after operation ,the average thickness of myelin sheath and the average number of myelinated axons were 0.779±0.475 μm、2 024±1 999( n =11)in experimental group and 0.413±0.132 μm、368±171( n =8)in contol sides respectively. There was significant difference between the experimental sides and control sides( P < 0.05). Eight weeks after operation, the regenerated nerve appeared more mature. There were significant difference in the average diameters, the thickness of myelin sheath and the number of myelinated axons between the experimental and control sides( P <0.05). Conclusion NGF within a silicone chamber enhanced facial nerve regeneration in New Zealand rabbits.
出处
《中华耳鼻咽喉科杂志》
CSCD
1998年第1期27-29,共3页
Chinese Journal of Otorhinolaryngology
基金
国家教委博士点基金
关键词
面神经
神经生长因子
神经再生
Facial nerve Nerve growth factors Nerve regeneration