缺氧缺血性脑病新生鼠胃壁内一氧化氮的改变

Changes in gastric nitric oxide in neonatal rats with hypoxic ischemic encephalopathy

目的探讨缺氧缺血性脑病新生鼠胃壁局部一氧化氮（ＮＯ）的改变及窒息对消化系统的影响。方法采用二氢硫辛酰胺脱氢酶ＮＡＤＰＨ组织化学方法，检测２４只正常或缺氧新生鼠胃壁各层一氧化氮合成酶（ＮＯＳ）的分布变化。结果急性缺氧组与正常对照组相比，ＮＯＳ阳性产物无论在分布、染色深浅、纤维密度及ＮＯＳ阳性胞体数目上，差异均无显著意义（Ｐ＞０．０５）。但在缺氧缺血性脑病组，其肌层的ＮＯＳ阳性纤维无论是密度还是染色深浅，均明显强于正常对照组，ＮＯＳ阳性胞体亦明显多于正常对照组，其差异有非常显著意义（Ｐ＜０．０１）；而粘膜和粘膜下层的ＮＯＳ分布与正常对照组相比，差异无显著意义（Ｐ＞０．０５）。

Objective To study the changes in nitric oxide (NO) in gastric wall in neonatal rats with hypoxic ischemic encephalopathy (HIE) and the influence of asphyxia on digestive system. Methods NADPH distribution of histochemistry analysis was taken to show the distribution of nitric oxide synthase (NOS) in the gastric wall of 24 neonatal rats. Results There was no significant difference in the number of NOS positive neuronal bodies and the density of NOS positive fibers between acute hypoxic rats and the normal controls ( P >0.05). However, in HIE neonatal rats, the NOS positive reaction products were markedly increased in the gastric muscle ( P <0.01) but not in the mucosal and submucosal layers compared with the control rats ( P > 0.05). Conclusion Decreased gastric motivation and the gastric mucosal lesion caused by asphyxia are associated with the changes in NO in gastric wall.