多发性肌炎/皮肌炎患者血清肿瘤坏死因子α水平及临床关系的观察

Study on the Serum L evel of Tumor Necrosis Factor-α and Its Relationship with Some Clinical Param eters in Polym yositis/Dermatom yositis

目的 探讨肿瘤坏死因子 (TNF)与多发性肌炎 /皮肌炎 (PM/DM)发病机理的关系。方法 用双抗体夹心 EL ISA法检测了 2 4例 PM/DM患者和 18例正常人 TN Fα水平 ,并分析了其与临床相关实验室指标的关系。结果 18例正常人血清中未测出 TN Fα,而 2 4例患者中 9例 TN Fα水平升高。可测出TNFα的 PM/DM患者组与未测出患者组比较前者具有 :1高尿肌酸水平 (P<0 .0 1) ;2低尿肌酐水平 (P<0 .0 5 ) ;3血沉高 (P<0 .0 1) ;血清肌酶两者之间未见明显差异。结论 TNFα水平与 PM/DM的活动性相关 ,TNFα可能在 PM/DM的发病机理中起着重要作用。

Objective To study the relationship between tumor necrosis factor(TNF ) and the pathogenesis of polymyositis/dermatomyositis(PM/DM) .Methods The serum levels of TN F-α were de- term ined in 2 4patients with PM/DM and 18norm al controls by double antibody sandwich EL ISA m ethod, and then their relationship w ith som e clinically related laboratory param eters w as analyzed.Results The results showed that the TNF -α was not detected in the control group,and the level of TNF -α was in- creased in9of2 4patients with PM/DM.In com parison with the group with elevated TNF-α to the group w ithout,we found the group w ith elevated TNF-α had:(1) a higher level of urinary creatine(P<0 .0 1) . (2 ) a lower level of urinary creatinine(P<0 .0 5 ) . (3) increased level of ESR (P<0 .0 1) . (4) no differ- ence in serum levels of CPK,L DH and A ST.Conclusion Our results indicate that there is a relationship between TNF -α level and the disease activity of PM/DM,and TNF -α m ay play an im portant role in the pathogenesis of PM/DM.