人乳头状瘤病毒与宫颈癌生物学行为的相关性研究

Relationship between human papillomavirus and cervical carcinoma

目的探讨高危型人乳头状瘤病毒（HPV）DNA与宫颈癌生物学行为的相关性。方法对66例宫颈癌患者及103例宫颈癌行手术治疗后随访病例宫颈脱落细胞，采用第二代杂交捕获技术行高危型HPV DNA检测。结果①66例中62例HPV DNA检测阳性，阳性率达94％，仅4例阴性（6％）。②宫颈癌患者各临床分期、病理分级间HPV DNA阳性率差异无统计学意义，但发生宫颈间质侵入的宫颈癌患者HPV阳性率及病毒DNA载量均明显上升，差异有统计学意义（P〈0．05）。③96％（99／103）的宫颈癌经手术治疗后患者HPV DNA检测为阴性。结论高危型HPV感染可能与宫颈癌血管生成、浸润和转移有关；第二代杂交捕获技术为高效的宫颈癌筛查方法。

Objective To explore the relationship between high-risk human papillomavirus （HPV） DNA and biological behavior of cervical carcinoma. Methods Sixty-six patients of cervical carcinoma with cytological examinations and 103 patients of cervical carcinoma followed-up after surgical operation were selected for high-risk HPV DNA test with second-generation hybrid capture technique （ HC2 Ⅱ ）. Results ①HPV DNA was positive in 62 and negative in four of 66 patients of cervical carcinoma with an overall prevalence of 94%. ②There was no significant difference in positive HPV DNA of patients with cervical carcinoma between their varied clinical stages and pathologic grades. But, HPV positivity and HPV DNA load in patients with myometrial invasion were higher than those in patients without invasion （ P 〈 0. 05 ）. ③ HPV DNA conversed to negative in 99 of 103 patients （96%） with cervical carcinoma after surgical operation from positivity before operation. Conclusions High-risk HPV infection may correlate with angiogenesis, invasion and metastasis of cervical carcinoma and HC2 Ⅱ can be used as an effective method to detect HPV DNA.