乳腺癌组织中Snail蛋白表达与肿瘤侵袭力的研究

Expression of Snail and tumor invasiveness in breast carcionma

应用免疫组织化学方法检测60例乳腺肿瘤和正常乳腺组织中Snail蛋白的表达；并将携带反义Snail的腺病毒载体（Adv-anti—Snail）转染高转移潜能的乳腺癌细胞株。Snail蛋白在正常乳腺组织中不表达或呈弱阳性，而在乳腺癌组织中呈胞核或胞浆的强阳性表达。Boyden小室体外侵袭实验提示转染Adv—anti—Snail的乳腺癌MDA—MB-231细胞的体外侵袭百分数为（10．5±1.3）％，而转增强型绿色荧光蛋白组的侵袭百分数为（68．2±2．1）％。提示Snail蛋白的表达升高与乳腺癌转移相皂，针对Snail的Adv-anti-Snail能逆转癌细胞的转移潜能。

Immunohistochemistry was used to detect the protein expression of Snail in 5 specimens jusxta-cancerous normal breast tissues, 35 specimens of cancerous tissues without metastasis and 20 specimens of breast carcinoma with lymphonode metastasis. Breast carcinoma cell line MDA-MB-231 was transfected with antisense Snail. Results showed that the expression of Snail protein was significantly higher in breast carcinomas than in their normal tissues. The mRNA and protein expressions of Snail in the breast carcinoma cells treated with antisense Snail was significantly decreased while the E-cadherin protein significantly increased （ both P 〈 0. 05 ）. The number of invasive cells treated with antisense Snail was （ 10. 5 ±1.3 ） % , while in treated with EGFP was （ 68. 2±2. 1 ） % （ P 〈 0. 05 ）. The abnormal expression of Snail contribute to the invasiveness of breast carcinoma. The antisense Snail could prevent the cells ability to invade in vitro, and the effect is related with the up-regulated E-eadherin protein.