摘要
用高效毛细管电泳测定了重组人促红细胞生成素（ｒｈＥＰＯ）的微多相性、肽图，同时采用毛细管电泳质谱联用技术鉴定了部分非糖基化胰酶消解片段和Ｏ－１２６糖基化肽的结构。方法简便、快速，适用于ｒｈＥＰＯ半成品的质量控制。
The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin (rhEPO) were analyzed by high performance capillary electrophoresis. In addition, some nonglycosylated tryptic fragments and O-126 glycosylated peptides were characterized by capillary electrophoresis coupled with electrospray-ionization mass spectrometry. The method developed in this paper was rapid, simple and especially suitable to the quality control of rhEPO raw material.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第3期249-253,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金重点资助项目。
关键词
毛细管电泳
人红细胞生成素
EPO
肽图
RHEPO
Capillary electrophoresis, capillary electrophoresis coupled with electrosprayionization mass spectrometry, recombinant human erythropoietin, microheterogeneity, tryptic mapping
