先天性积水肾脏组织中水通道蛋白mRNA表达与肾实质厚度变化的相关性研究

Correlation between aquaporins mRNA expression and renal parenchyma thickness in congenital hydronephrotic kidney in children

目的观察先天性肾积水。肾组织水通道蛋白1～4（AQP1～4）mRNA表达及与肾实质厚度变化之间的关系。方法采用逆转录聚合酶链反应法检测37例先天性。肾盂输尿管连接处狭窄患儿[年龄（60．3±48．8）个月]和6例正常对照组[年龄（62．7±17．1）个月]肾组织标本中AQP1～4mRNA的相对表达量。术前B超检测积水肾实质厚度，并与术中测定结果比较。AQP1～4mRNA相对表达量与肾实质厚度之间进行相关性分析。结果积水肾组织中AQP1～4mRNA表达量分别为0．39±0．22、0．42±0．20、0．52±0．22、0．30±0．18，正常对照组分别为0．90±0．10、0．92±0．09、0．98±0．12、0．74±0．21，2组比较差异均有统计学意义（P〈0．01）。B超测定积水肾实质厚度平均（5．01±2．38）mm，肾盂放水后肾实质厚度平均（5．12±1．81）mm，差异无统计学意义（P〉0．05）。梗阻肾脏实质厚度与AQP1～4mRNA相对表达量呈正相关（r分别为0．773、0．772、0．557、0．625，P〈0．01）。结论积水。肾实质厚度变化与AQP1～4mRNA表达量下降有一定相关性。

Objective To investigate the relationship between aquaporin-1, -2, -3, -4 mRNA （AQPI 4） and renal parenehyma thickness in congenital hydronephrotic kidney in children. Methods The expressions of aquaporin 1, -2, 3, and-4 mRNA in hydronephrotie kidney of 37 children （aged 60.3±48.8 months） were evaluated with congenital hydronephrosis and control kidney of 6 children （aged 62.7 ± 17.1 months） by using semi quantitative reverse transcriptase polymerase chain reaction technique. Hydronephrotic kidney parenchyma thickness was measured by B-Ultrasound preoperatively and verified at operation. The relations of aquaporin 1, -2, -3, and 4 mRNA to the hydronephrotic kidney parenchyma thickness were analyzed by correlation analysis. Results The aquaporin 1 ,-2,-3, and -4 /beta-actin ratio in the hydronephrotic kidney and normal kidney were 0. 39 ± 0. 22 vs 0.90± 0.10, 0. 42±0. 20 vs 0. 92±0. 09, 0.52±0.22 vs 0. 98±0.12, 0.30±0. 18 vs 0.74±0.21 respectively, and the differences were significant （P〈0.01）. Hydronephrotic kidney parenchyma thickness measured by B-Ultrasound was 5.01± 2.38 ram, which was identical with those measured at operation. Significant correlation was found between the levels of aquaporin 1,-2,-3, and 4 mRNA and hydronephrotic kidney parenchyma thickness （r 0. 773, 0. 772, 0. 557, 0. 625, respeetively; P〈0.01）. Conclusions Significant correlation exists between decreased expressions of aquaporin 1 ,-2, -3, and -4 mRNA and atrophic change of renal parenchyma. This result may provide evidence to explain the mechanism why the thinner renal parenchyma thickness, the weaker renal concentration and dilution function.