盐和PEG胁迫对丝瓜幼苗抗氧化酶活性及丙二醛含量的影响

Effect of salt and PEG to antioxidant enzymes activity and MDA concentration of Luffa cylindrical Roem

测试不同浓度NaCl和PEG胁迫条件下丝瓜幼苗体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性及丙二醛(MDA)、可溶性糖(SS)、叶绿素含量的变化情况。结果表明,在两种胁迫处理条件下,三种抗氧化酶活性均高于对照;MDA含量随NaCl处理浓度的增加先上升后下降,PEG胁迫条件下MDA含量随处理浓度的增加而下降;可溶性糖(SS)含量随NaCl处理浓度的增加先上升后下降,随PEG处理浓度的增加明显上升;两种处理条件下叶绿素含量均随处理浓度增加而下降。

The activity of anti-oxidant enzymes（Superoxide dismutase（SOD）,Peroxidase（POD）,Catalase（CAT））,parameters of oxidative stress malondialdehyde（MDA）,soluble sugar（SS） and chlorophyll content of shoots were investigated in L.cylindrical.The seedlings of L.cylindrical were treated with varying NaCl（0,50,100,150 mmol/L） and PEG（0,10%,15%,20%） stress.The activities of SOD,POD and CAT showed different developing tendencies with the increase of the concentration of NaCl or PEG in shoots of L.cylindrical,but it was higher under stress conditions than control.MDA concentration of shoots increased than decreased under salt stress condition,but it was higher under stress conditions than control,MDA concentration of shoots decreased under PEG stress condition.SS concentration of shoots increased than decreased under salt stress condition,but it increased with PEG concentration increased obviously.Chlorophyll content of shoots decreased under stress conditions.The stress tolerance of L.cylindrical under salt and PEGr stress condition are probably due to its ability to exhibit high SOD,POD and CAT enzyme activities and osmotic adjust action of soluble sugar.