摘要
目的研究不育男性精子染色质结构参数与精液常规参数的关系。方法采集36例男性不育患者和18例健康对照者的精液标本,用TUNEL法检测精子DNA碎片化,CMA3染色法检测精子染色质包装质量,按照世界卫生组织标准(1999)检测精液常规参数。结果不育组的精子TUNEL阳性率和CMA3阳性率均显著高于健康对照组,差异具有统计学意义(P<0.05),精液常规参数正常的不育男性精子TUNEL阳性率和CMA3阳性率也显著高于健康对照组,差异具有统计学意义(P<0.05),精子TUNEL阳性率和CMA3阳性率与精液常规参数之间具有显著的相关性(P<0.05)。结论不育男性的精子染色质结构检测结果与健康男性存在显著差异,精子染色质结构检测可提供反映男性生育能力的信息。
Objective To analyze the relationship between sperm chromatin structure and semen conventional parameters of infertility men. Methods Semen samples were collected from thirty-six infertility male patients and eighteen healthy donors. Sperm DNA fragmentation was measured with TUNEL and sperm chromatin packaging was measured with CMA3 staining. Semen conventional analysis was performed based on WHO criteria (1999). Results Positive rates of TUNEL and CMA3 staining in the infertility group were significantly higher than those in the control group. Positive rates of TUNEL and CMA3 staining in the subgroup of infertility patients with normal semen parameters were also significantly higher than those in the control group. Significant correlations were found between semen conventional parameters and TUNEL positive rate or CMA3 positive rate. Conclusion The sperm chromatin structure of infertility men was significantly different from those of healthy men. Analysis of sperm chromatin structure would be helpful for judgement of male reproductive capability.
出处
《中国男科学杂志》
CAS
CSCD
2009年第3期29-32,共4页
Chinese Journal of Andrology
关键词
不育
男性
精子
染色质
精液
infertility, male
spermatozoa
chromatin
semen