抗生素敏感性微生物源追踪方法建立

Study on antibiotic resistance analysis with microbial source tracking method

目的建立抗生素敏感性微生物源追踪方法,以分析淮河流域某水库粪便污染来源。方法采集水库周边已知来源粪便标本,分离指示菌,接种至含抗生素的培养基,筛选能区分不同来源指示菌的抗生素浓度,用JMP7.0软件进行判别分析检验效果。同时采集水样进行粪便污染来源追踪。结果筛选的不同抗生素及其浓度能将已知来源指示菌正确归类率分别为93.05%(2分类)、85.75%(3分类)和78.53%(9分类)。100株指示菌中有58株来自家畜,25株来自家禽,11株来自狗,4株来自人,2株来自野生动物。水样标本中主要的粪便污染来源为猪、牛、鸡,分别占水样分离指示菌总数的36%,17%和15%。结论所筛选的抗生素浓度均能较好地区分指示菌的不同来源,该方法的建立可以为查找水体粪便污染来源以及水体治理提供参考与帮助。

ObJective To set up an antibiotic resistance analysis with microbial source tracking method and track the source of the fecal pollution in a reservoir of the Huaihe River. Methods Fecal samples were collected from known pollution sources around the reservoir and indicator bacteria isolated from the samples were transferred onto the Trypticase Soy Agar plates with different antibiotics. The proper concentrations for the growth of the indicator bacteria were discriminated with JMP 7.0 software. Water samples were collected for pollution source tracking. Results The average rates of correct classification from the selected concentrations of antibiotics were 93.05% （two sources）, 85.75% （three sources） and 78.53% （ nine sources）. The fecal pollution in the water samples were mainly from swine ,cow and chicken with the rate of 36%, 17% and 16% ,respectively. Conclusion The selected concentrations of antibiotics can be used to find the sources of the indicator bacteria. Antibiotic resistance analysis with microbial source tracking method can be applied to track the source of water bacterial pollution.