根围细菌次生代谢物的生物活性筛选

Bioactivity Screening for Secondary Metabolite of Rhizosphere Bacteria

从全国7个地区采集的植物根围土壤样品中分离得到1700余株植物根围细菌。采用室内离体筛选的试验方法,以5种植物病原细菌作为靶标,筛选研究得到有生物活性的菌株840株。其中10%的菌株对5种植物病原细菌都具有拮抗活性,其余菌株则仅对1种或几种病原细菌具有拮抗活性。从中进一步筛选得到对小麦白粉病也具有防治效果的菌株,其防治效果最高可达60%。将最终获得的同时对病原细菌有拮抗效果和真菌病害有防治效果的根围细菌作为以后深入研究的对象。

Collect rhizosphere soil sample from 7 different region in China, then obtain 1700 rhizosphere bacteria through separate bacteria from different soil sample. In vitro, evaluate the bioactivity of rhizosphere bacteria against five varieties pathogenic bacteria. At last, we obtain 840 rhizosphere bacteria against pathogenic bacteria. Only 10% rhizosphere bacteria had bioactivity against all of the 5 pathogenic bacteria, the others had not the character. These 840 rhizosphere bacteria as resource, screen the bioactivity against the wheat powder mildew. The best control effect against the wheat powder mildew achieve 60%. Select the rhizosphere bacteria have bioactivity against pathogenic bacteria and the wheat powder mildew as farther research object.