针叶树生散斑壳属部分种内及近似种间亲缘关系的ISSR分析

ISSR Analysis of Relationship of Partial Intraspecific and Approximate Interspecific Lophodermium Strains from Conifer Trees

应用ISSR分析技术,对22个散斑壳属(Lophodermium Chevall)菌株进行种内和近似种间亲缘关系的分析。通过欧氏最短距离中的类平均法聚类,得到各菌株之间的遗传距离在5.93～7.41之间,表明种内及近似种间有较丰富的遗传多样性。另外,在遗传距离7.12处,将22个供试菌株分为5类。通过ISSR分析结果结合表型性状的比较和分析,论证了子囊果埋生位置,子座、唇、子囊、子囊孢子等形态学特征以及寄主种类差异等在该类菌物种水平分类上的重要性。

Relationship of 22 Lophodermium strains were analyzed applying inter-simple sequence repeat （ISSR） technique. The results showed that the genetic distance among the strains was 5.93 - 7.41 by UPGMA, indicating that they have relatively rich of genetic diversity among intraspecific and approximate interspecific strains. In addition, the 22 tested strains were classified into 5 groups in the position of 7.12 genetic distances. Comparison and analysis of the ISSR analytic results combined with phenotypic characteristics demonstrated the importance of differences and so on of some morphological characteristics in the classification of the kind of fungi in specific level, such as the embedment position of ascomata, stromata, labia, asci, and ascospores and categories of the hosts.