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含CpG序列PRRSV ORF5重组质粒在小鼠细胞中的免疫

Cellular Immune Response Evicited Eukaryotic Expression Vector Containing PRRSV ORF5 and CpG Motifs
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摘要 为了提高猪繁殖与呼吸综合征病毒(PRRSV)基因免疫的效果,将成功构建含CpG序列PRRSVORF5的真核重组表达质粒CpG-pVAX1-ORF5免疫SPF小鼠,检测小鼠的脾T淋巴细胞亚群数量(CD4+和CD8+)以及淋巴细胞增殖反应(MTT法)水平。结果表明,构建的含CpG序列真核重组表达质粒CpG-pVAX1-ORF5能诱导小鼠产生针对PRRSV的细胞免疫。 To improve the effect of the gene immunization against porcine reproductive and respiratory syndrome virus(PRRSV)we constructed the eukaryotic expression vector CpG-pVAX1-ORF5 containing PRRSV ORF5 gene coding region and CpG motif.The recombinant expression plasmid was inoculated into SPF mice.The number of CD4+,CD8+ and the lymphocyte proliferation test(MTT) in peripheral blood of mice vaccinated were used to detect.The results showed that the recombinant plasmid CpG-pVAX1-ORF5 could induce higher response of cellular immune response.
出处 《西北农业学报》 CSCD 北大核心 2009年第2期1-5,共5页 Acta Agriculturae Boreali-occidentalis Sinica
基金 山东省自然科学基金项目资助(编号:Z2003D01)
关键词 猪繁殖与呼吸综合征病毒 ORF5基因 细胞免疫应答 Porcine reproductive and respiratory syndrome virus(PRRSV) ORF5 gene Cellular immune response
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