几种miniprep提取质粒DNA方法的比较与改良

A comparative and improved study on plasmid DNA extraction

目的比较并建立一种简单的、稳定的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法采用琼脂糖凝胶电泳对质粒DNA提取的试剂盒法、煮沸法、碱裂解法、碱裂解结合酚氯仿法四种方法进行比较。结果试剂盒法的电泳谱带亮度最高且无RNA条带,煮沸法、碱裂解法、碱裂解结合酚氯仿法的电泳谱带亮度依次增高。结论试剂盒法制备DNA纯度最高,且操作简便省时。

Objective To explore a simple,improved and steady method for extraction of plasmid DNA and its value in molecular biological research. Methods Four routine DNA extractions of plasmid were compared by using agarose gel electrophoresis. Results The band using TIANprep Mini Plasmid Kit was the brightest without RNA band.The brightness of the bands increased using boiling lysis method,alkaline lysis method,alkaline lysis method combined with phenol-chloroform extraction method in turns. Conclusion Lysis by TIANprep Mini Plasmid Kit is simple and convenient for producing a high purity of plasmid DNA.