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醇引起酵母醇脱氢酶构象变化的光谱研究 被引量:4

SPECTRAL STUDY OF THE CONFORMATIONAL CHANGE OF YEAST ALCOHOL DEHYDROGENASE INDUCED BY ALCOHOL
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摘要 应用紫外、荧光、CD谱研究了酵母醇脱氢酶经乙醇、正丙醇和乙二醇作用后的构象变化。结果表明220nm、280nm处的紫外吸收,336nm的相对荧光强度随醇浓度增大而加强。CD谱显示乙醇、乙二醇引起酶分子在208nm、220nm处的双负峰逐渐加强,正丙醇使220nm处负峰加强,208nm处负峰减弱并红移,直至完全消失。上述数据表明醇浓度增加导致酶分子结构逐步松散,同时伴随着活力的丧失。 The conformational change of Yeast Alcohol Dehydrogenase (YADH) at the different concentration of ethanol,npropanol and ethylene glycol was studied by means of ultraviolet spectrum,fluorescence spectrum and circular dichroism spectrum.The results showed that the ultraviolet absorbance at 220nm and 280nm as well as the relative fluorescence intensity at 336nm of YADH increased with increasing alcohol concentration.The negative peakes at 208nm and 220nm of YADH in circular dichroism spectrum with the solvent of ethanol,ethylene glycol were obviously intensified,but the 220nm peak of YADH was increased in the presence of npropanol while the 208nm peak was decreased and red shifted in position as to completely lost.According to the data above,it indicates that the conformation of YADH was changed with losing activity at the various concentration of alcohol.
作者 张强 袁静明
出处 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 1998年第1期30-33,共4页 Spectroscopy and Spectral Analysis
关键词 醇脱氢酶 构象 光谱 酵母酵脱氢酶 Alcohol dehydrogenase, Alcohol, Conformation, Spectrum
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  • 1袁静明,山西大学学报,1989年,12卷,2期,209页
  • 2杨缜,Biocatalysis in non-conventional media,1992年
  • 3杨缜,博士学位论文,1992年

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