心得宁的丹磺酰氯衍生-室温磷光分析

ROOM TEMPERATURE PHOSPHORIMETRIC DETERMINATION OF PRACTOLOL WITH DANSYL CHLORIDE LABELING

心得宁的丹磺酰氯衍生物具有强的荧光及磷光，可用于心得宁的发光分析。本文详细研究了心得宁同丹磺酰氯衍生的反应条件及检测衍生物的最佳光谱条件。利用一阶导数光谱技术，无需对衍生物溶液进行任何分离，实现了心得宁的室温磷光检测。

Dansyl Chloride (DNSCl)was used as a phosphorescent labeling agent for the determination of practolol.The derivative was formed by refluxing practololethyl acetate solution with the solid DNSCl in the presence of potassium carbonate anhydride.Roomtemperature phosphorescent (RTP)properties of the derivative were studied by a micellestabilized roomtemperature phosphorescence (MSRTP) method.Phosphorescence lifetimes of practololDNS and the DNSCl were found to be 0791 and 0408ms,respectively.By applying the derivative MSRTP technique,practolol could be quantitatively determined without previous separation process.Detection limit of practolol with the proposed method was 019μg/mL.