^(31)P核磁共振观察γ-干扰素对Molt-4细胞作用后含磷代谢物及pH变化

31P-NMR Observation on Changes in Phosphorus Containing Metabolite and pH after Action of Interferon_γ on Molt_4 Cells

Ｍｏｌｔ＿４细胞的３１Ｐ核磁共振谱由磷酸单酯、无机磷（Ｐｉ）、双磷酸双酯、三磷酸腺苷（ＡＴＰ）的共振峰组成。用３１Ｐ核磁共振观察了γ－干扰素对Ｍｏｌｔ＿４细胞作用后含磷化合物的变化。根据Ｐｉ的化学位移对Ｍｏｌｔ＿４细胞内ｐＨ进行了监测。３１Ｐ核磁共振能一次同时监测细胞内多种含磷代谢物，是一种无损伤研究细胞代谢的有效方法。

31P-NMR spectrum of Molt_4 cells consists of resonance peaks of phosphomonoester,inorganic phosphate (Pi),diphosphodiesters and ATP. Changes in phosphorus containing compound after action of interferon_γ on Molt_4 cells were observed by 31P-NMR spectrum. The pH in intra_molt_4 cells were monitored based on chemical shift of Pi. Several intracellular phosphorus containing compounds can be simultaneously monitored by 31P-NMR.