以30日龄长爪沙鼠为介体建立犬贾第虫纯培养

The Establishment of Axenic Cultivation of Giardia Canis with 30-day-old Gerbls

将取自然感染犬贾第虫的德国牧羊犬的包囊利用蔗糖梯度离心-G1耐酸漏斗滤过法进行分离和纯化,经口感染给30日龄的沙鼠.8d后无菌取小肠上段,分离出滋养体,转入改良TYI-S-33培养基中37℃培养.72h后在管壁上形成了细胞单层,将培养物置肉汤及血琼脂平板培养,未见细菌及霉菌污染,为纯培养.

The cysts of Giardia canis were isolated and purified by saccharosedensity gradient centrifugation GI funnel,and the 30-day old sucking gerbls were infected with the purified cysts through orute.8 days after infeetion,trophozoites were isolated aseptically from the superor segment of small intestine of infected animals.Then the samoles were inoculated inti modified TYI-S-33 medium and cultivated at 37℃.72h after the parasites which gradually adapted to environment and formed a cellular monolayer on the inner surface of culture tubes.No contamination with bacteria or fungus was found,indicating it was an axenic culture.