鸡肉中三聚氰胺残留量检测方法研究

Study on Detecting Methods on Residual Amount of Melamine in Chicken Meat

[目的]为检测动物性食品中的三聚氰胺提供参考方法。[方法]以鸡肉匀浆为材料，用高效液相色谱法和气相质谱法检测其中的三聚氰胺含量，并对检测方法的回收率和精密度进行测定。[结果]高效液相色谱法确定的鸡肉中三聚氰胺的最低检测限为1μg／g，最低定量限为2μg/g，在0．05-50μg范围内线性关系良好；方法的回收率大于80％，测定结果的日内变异系数小于7％，日间变异系数小于10％。气相质谱法确定的三聚氰胺的最低检测限为30ng／g，最低定量限为5030ng/g，在5—500ng／g范围内线性关系良好；方法的回收率在80％以上，测定结果的日内变异系数小于8％，日间变异系数小于10％。[结论]该研究为鸡肉中三聚氰胺的检测提供了新方法。

[ Objective ] The study was to provide a reference method for detecting melamine in animal food. [ Method ] With the homogenate of chicken meat as the material, the melamine amount in it was detected with high performance liquid chromatography （ HPLC ） and gas phase mass spectrometry method, and then the recovery rate and the precision of the 2 detection methods were determined. [ Result] The minimum detection limit on melamine in chicken meat by high performance liquid chromatography （HPLC） was 1 μg/g, and the limit quantification was 2 μg/g, and in 0. 05 -50 μg/g, the linear relationship was good. The recovery rate of this method was over 80%, the within-day variation coefficient was less than 7%, and the inter-clay variation coefficient was less than 10%. The minimum detection limit on melamine in chicken meat by gas phase mass spectrometry method was 30 ng/g, and the limit quantification was 5 030 ng/g, and in 5 -500 ng/g, the linear relationship was good. The recovery rate of this method was over 80%, the within-day variation coefficient was less than 8%, and the inter-day variation coefficient was less than 10%. [ Conclusion]The study provided a new method to detect melamine in chicken meat.