高效液相色谱法测定扭盔马先蒿中毛蕊花苷的含量

Determinati on of Verbascoside in Pedicularis davidii by HPLC

[目的]建立HPLC法测定扭盔马先蒿中毛蕊花苷含量的方法。[方法]采用HPLC法Kromasil C18柱(250 mm×4.6 mm,5μm),乙腈-1%冰醋酸溶液(1∶4)为流动相,在330 nm波长下测定样品中毛蕊花苷的含量。[结果]该方法专属性强,平均加样回收率为96.87%,RSD值为2.18%(n=5)。[结论]该方法方便,分离效果好,适用于扭盔马先蒿的鉴别和质量的控制。

[ Objective] To establish an analytical method for the determination of verbaseoside in Pedicularis davidii by HPLC. [ Method ] By using HPLC, the conditions of chromatography： Kromasil C18（250 mm × 4.6 mm, 5μm ） was used with a mobile phase of CH3CN - 1% CH3COOH（1：4） ,to determine verbaseoside content in the sample with wavelength 330 nm. [ Result] With high specificity, the average spiking recovery rate was 96.87% , RSD value was 2.18% （ n = 5 ）. [ Conclusion ] This method was convenient and had an ideal result in the separation, which could be adopted in the identification and quality eontrol of Pedicularis davidii.