三系杂交稻亲本随机扩增多态性DNA(RAPD)分析

RAPD Studies of Parents of Three-Line Hybrid Rice

选用９个随机引物对３１份杂交水稻亲本材料进行了ＲＡＰＤ分析，共检测到６０条多态性带。聚类分析结果表明，所有供试材料可以被明确地区分。在９个随机引物中，有８个具有较高的多态性检测能力。以这８个引物为基础，选用任两个引物即可在任一对材料中检测出多态性的频率在９６１３％以上，而选用任３个引物则该频率在９９２１％以上。这显示了运用ＲＡＰＤ鉴定稻种具有简便、灵敏、高效的优点，在鉴定杂交稻种的实践中有着良好的应用前景。

Seven rice male sterile lines, 12 maintainer lines and 12 restorer lines were analyzed by RAPD with 9 primers Altogether, 118 fragments were generated, of which 60 detected polymorphisms among rice lines tested. Cluster analysis showed that all lines could be uniquely distinguished by at least one RAPD marker. Eight of nine primers can detect high polymorphism. The frequencies of polymorphism in any pairs of lines would be higher than 96.13% when any two of the 8 primers were used. If any three of the 8 primers were used, the frequencies would be higher than 99.21%. The eight primers were therefore recommended as candidates for the identification of hybrid rice seeds.