血小板生长因子(PDGF-BB)刺激体外培养兔肺动脉平滑肌细胞DNA和蛋白质合成

PDGFBB STIMULATES DNA AND PROTEIN SYNTHESIS IN PULMONARY ARTERIAL SMOOTH MUSCLE CELLS OF RABBIT IN VITRO

应用细胞培养、３Ｈ－ＴｄＲ和３Ｈ－Ｌｅｕｃｉｎｅ掺入方法，观察血小板生长因子ＢＢ（Ｐｌａｔｅｌｅｔ－ｄｅｒｉｖｅｄＧｒｏｗｔｈＦａｃｔｏｒＢＢ）对体外培养兔肺动脉平滑肌细胞ＤＮＡ和蛋白质合成的影响。结果表明：（１）当ＰＤＧＦ－ＢＢ浓度为１０ｎｇ／ｍｌ时，３Ｈ－ＴｄＲ掺入值已较对照组显著增高（６２６２．５±４１２．９ｖｓ８３３．５±１２４．０，Ｐ＜０．０５）；当ＰＤＧＦ－ＢＢ浓度为２０ｎｇ／ｍｌ时，３Ｈ－Ｌｅｕｃｉｎｅ掺入值亦较对照线显著增高（１０２１２．８±６３８．３ｖｓ７３４０．３±１１９７．９，Ｐ＜０．０５）。（２）ＰＤＧＦ－ＢＢ浓度在５－２５ｎｇ／ｍｌ范围内，３Ｈ－ＴｄＲ，３Ｈ－Ｌｅｕｃｉｎｅ掺入值与剂量直线相关（ｒＤＮＡ＝０．９７，ｒｐｒｏｔ＝０．９０Ｐ＜０．０５）。说明ＰＤＧＦ－ＢＢ刺激体外培养兔肺动脉平滑肌细胞ＤＮＡ和蛋白质合成。

? To assess the effects of plateletderived growth factor(PDGFBB)on DNA and protein synthesis in pulmonary arterial smooth muscle cells (PAMC) of rabbit.3HTdR and 3 HLeucine incorporation were performed in the 3-5th passage of cultured rabbit PASMC(4.5×104/well).Results:(1)When 10ng/ml of PDGFBB was added,3HTdR incorporation significantly increased as compared with control (6262.5±412.9vs833.5±124.0,P<0.05);3 HLeucine incorporation increased significantly (10212,8±638.3vs7340.3±1197.9,P<0.05)as the concentration of PDGFBB was 20ng/ml.(2)There was a dosedependent response with linear regression of both 3 HTdR or 3 HLeucine incorporation into PASMC as PDGFBB ranged from 5 to 25ng/ml (rDNA=0.97,rPROt=0.90 P<0.05).Conclusion:PDGFBB promoted proliferation of the PASMCs。