日本沼虾三种细胞器在精子发生过程中变化的研究

STUDIES ON CHANGES IN THREE ORGANELLE DURING SPERMATOGENESIS OF MACROBRACHIUM NIPPONENSE (DE HAAN)

应用透射电镜技术，研究了日本沼虾在精子发生过程中线粒体、溶酶体及内质网的变化．从精原细胞到精子，生精细胞内线粒体数量逐渐增多，结构渐趋复杂化，并形成衍生物，参与精子顶体的形成．溶酶体从初级溶酶体逐渐形成次级溶酶体，其中包括包裹溶酶体及溶噬体；部分溶酶体构成精子顶体的一部分．精子发生的全过程中，未见典型的粗面内质网（ＲＥＲ），仅见泡状粗面内质网（ＶＲＥＲ）；滑面内质网只在精原细胞期出现．支持细胞的作用包括两个方面：一为支持作用，使生精细胞有一个适宜的发育场所；二为营养作用，为生精细胞的发育提供足够的物质和能量．

The changes of mitochondria, lysosomes and endoplasmic reticulum during spermatogenesis of Macrobrachium nipponense (de Haan)were investigated by the method of transmission electron microscope(TEM). From spermatogonium to sperm, mitochondria in spermatogenic cells gradually increased in number, with complicated structure , then formed derivatives, took part in formation of acrosome. The forms of lysosome changed from primary lysosome to second lysosome, including the formation of wrapping lysosome and lysosomophagosome and some lysosomes became one part of acrosome. Typical rough endoplasmic reticulum(RER) were not seen during the process of spermatogenesis, only vesicular RER appeared and smooth endoplasmic reticulum(SER) only presented at spermatogonium stage. The function of sustentacular cells was found in two aspects: one was sustentacular, which made a suitable place for the development of spematogenic cells, another was nutritive, which supplied materials and energy for the development of spermatogenic cells.