慢生花生根瘤菌的遗传多样性研究(英文)

STUDY ON THE GENETIC DIVERSITY OF PEANUT BRADYRHIZOBIAL STRAINS 

用ＲＥＰ１Ｒ１和ＲＥＰ２１ＤＮＡ为引物，对２２株四川土著慢生花生根瘤菌染色体ＤＮＡ的基因外回文重复序列（ＲｅｐｅｔｉｔｉｖｅＥｘｔｒｏｇｅｎｉｃｐａｌｉｎｄｒｏｍｉｃｓｅｑｕｅｎｃｅＲＥＰ）进行了ＰＣＲ扩增和凝胶电泳，并对电泳结果的指纹图型进行了相似性聚类分析．结果表明了四川土著慢生花生根瘤菌的遗传多样性．

The distribution of repetitive DNA(repetitive extragenic palindromic )sequences in the genomes of 22 peanut bradyrhizobial strains isolated from the different cultivars and locations in Sichuan was examined by using conserved primers corresponding to repetitive extragenic palindromic sequences and polymerase chain reaction(PCR).The fingerprint patterns of the resulting REP PCR products were visualized on 1.5% agarose gel and found to be strain specific. The dendrogram generated on the basis of the similarities of the REP PCR fingerprint patterns grouped the strains in correspondence with the isolation sites and cultivars.This result indicated that Sichuan peanut bradyrhizobia were genetically very diverse since 7 clusters were formed among the 22 strains tested.REP PCR technique is a useful tool for the studies on the diversity of those closely related rhizobial and bradyrhizobial strains.